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The ability to cryopreserve mammalian embryos has become an integral part of assisted reproduction, both in human and veterinary medicine. Despite differences in the size and physiological characteristics of embryos from various species, the embryos have been frozen by either of two procedures: The aim of our study was to compare the effect of slow freezing and vitrification to the chromatin structure, energy status and reactive oxygen species production of mouse morulae and blastocysts. Mouse morulae and blastocysts were randomly allocated into vitrification, slow freezing and control groups. Cryopreservation affected chromatin integrity at a greater extent at the morula than the blastocyst stage.